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234338

T1107 polymer expressed potential anti-tumor effects against HepG2 cell line

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Biochemistry

Abstract

Background: Polymeric nanoparticles (NPs) are obtained naturally, semi-synthetically or synthetically by a polymerization reaction. Tetronics based ethylene oxide-propylene oxide copolymers have gained a great of interest. It demonstrated interactions with cell membranes with potential for developing new biomaterials for application in nanomedicine . Aim: The primary aim of the current study is to test the direct anticancer activity of unmodified tetronic (T1107), P-HB+aminated tetronic and N, N DMAB+aminated tetronic polymersand the associated by apoptosis and cell cycle. Materials and Methods: Synthetic polymers were prepared and characterized to confirm the modification. Anti-tumor activity was examined in vitro using human hepatocellular carcinoma (HepG2) cell line.  Cell viability (MTT), cell cycle and apoptosis were evaluated by flow cytometry. Results: Unmodified tetronic (T1107) decreased HepG2 viability (by 30%) than untreated HepG2 cells.  Treatment with P-HB+aminated tetronic and N,N DMAB+aminated did not give a significant effect on Hepg-2 cells. Treatment of HepG2 with unmodified tetronic (T1107) induced cell cycle arrest at G0 phase (39.4%), while P-HB+aminated tetronic and N,N DMAB+aminated tetronic induced cell cycle arrest at G1 phase (by 54.8% and 48.2%, respectively as compared to treatment with the doxorubicin (DOX) as a reference drug, which induced Hepg-2 cell cycle arrest at GO phase (by 39.1%). The unmodified tetronic (T1107) increased the numbers of late apoptotic cells (by 49.4%), while P-HB+aminated tetronic and N,N DMAB+aminated tetronic  did not  induced significant apoptosis. Conclusion:.Unmodified Tetronic T1107 induces an anticancer effect more than modified Tetronic polymers.  

DOI

10.21608/jcbr.2021.92796.1227

Keywords

apoptosis, Cancer, Cell cycle, Tetronic, polymer

Authors

First Name

El-Refaie

Last Name

Kenawy

MiddleName

-

Affiliation

Chemistry Department, Faculty of Science, Tanta University, Tanta, Egypt.

Email

ekenawy@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Salem

MiddleName

Labib

Affiliation

Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt

Email

mohamedlabibsalem@yahoo.com

City

Tanta

Orcid

-

First Name

Noura

Last Name

Sanoh

MiddleName

-

Affiliation

Chemistry Department, Faculty of Science, Tanta University, Egypt. Center of Excellence in Cancer Research.

Email

noraelsaeed12@gmail.com

City

Tanta

Orcid

-

First Name

Mohamed

Last Name

Azaam

MiddleName

-

Affiliation

Chemistry Department, Faculty of Science, Tanta University.

Email

mmkazaam@yahoo.com

City

Tanta

Orcid

-

Volume

6

Article Issue

1

Related Issue

33715

Issue Date

2022-03-01

Receive Date

2021-08-26

Publish Date

2022-03-01

Page Start

1

Page End

10

Print ISSN

2682-261X

Online ISSN

2682-2628

Link

https://jcbr.journals.ekb.eg/article_234338.html

Detail API

https://jcbr.journals.ekb.eg/service?article_code=234338

Order

1

Type

Original Article

Type Code

885

Publication Type

Journal

Publication Title

International Journal of Cancer and Biomedical Research

Publication Link

https://jcbr.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023