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194075

The diagnostic and predictive values of miRNA-21, miRNA-126, miRNA-513a, and miRNA-98 in patients with non-small cell lung cancer (NSCLC)

Article

Last updated: 04 Jan 2025

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-

Tags

Inflammatory and cancer

Abstract

Background: The majority of lung cancer cases are non-small cell lung cancer (NSCLC). MicroRNAs (miRNAs) are small non-coding molecules that control target gene(s) expression. Aims: Identification of circulating levels of miRNA-21, 126, 513a, and 98 in NSCLC and assessment of their diagnostic and predictive values. Material and Methods: In this study, the levels of miRNA-21, 126, 513a, and 98 were evaluated in 19 NSCLC patients and 11 healthy individuals. miRNAs were extracted from patients and healthy control whole blood samples and cDNA was synthesized with the specific primers for each miRNA, real-time quantitative polymerase chain reaction (RT-qPCR) was used to assay the expression pattern of miRNAs. Results: The circulating levels of miRNA-21, 126, and 513a were significantly up-regulated in NSCLC patients as compared to the healthy subjects. Of interest, the circulating levels of these miRNAs were about twice higher in stage IV patients than stage III patients.  miRNA-513a showed the highest diagnostic accuracy (AUC = 0.942, specificity = 100, and sensitivity = 85.7) and sensitivity was improved when in combination with miRNA-21 (AUC = 0.929, specificity = 90.91, and sensitivity = 92.8). All studied miRNAs showed clear changes during the treatment of NSCLC patients with the platinum regimen. Conclusions: miRNA-21, 126, and 513a may be considered as candidate diagnostic circulating biomarkers in NSCLC as well as a potential predictive biomarkers for the response to the platinum regimen. miRNA-513a showed the highest diagnostic accuracy for distinguishing NSCLC patients from healthy controls.

DOI

10.21608/jcbr.2021.55228.1107

Keywords

Circulating miRNA, Diagnostic Biomarkers, Lung Cancer, NSCLC, platinum chemotherapies, predictive biomarkers, RT-qPCR

Authors

First Name

Sameh

Last Name

Nakhla

MiddleName

-

Affiliation

Department of Radiation Science, Medical Research Institute, Alexandria University, Alexandria, Egypt

Email

sameh.nakhlh@alexu.edu.eg

City

-

Orcid

0000-0003-3886-3795

First Name

Maher Kamel

Last Name

Kamel

MiddleName

-

Affiliation

Department of Biochemistry, Medical Research Institute, Alexandria University, Alexandria, Egypt.

Email

maher.kamel@alexu.edu.eg

City

-

Orcid

-

First Name

Sawsan

Last Name

Moussa

MiddleName

-

Affiliation

Department of Radiation Science, Medical Research Institute, Alexandria University, Alexandria, Egypt

Email

-

City

-

Orcid

-

First Name

Inas

Last Name

Ali

MiddleName

M.

Affiliation

Department of Cancer Management and Research, Medical Research Institute, Alexandria University, Alexandria, Egypt

Email

-

City

-

Orcid

-

First Name

Mostafa

Last Name

Elnaggar

MiddleName

-

Affiliation

Department of Cancer Management and Research, Medical Research Institute, Alexandria University, Alexandria, Egypt

Email

-

City

-

Orcid

-

First Name

Soha

Last Name

Talima

MiddleName

A.

Affiliation

Department of Clinical Oncology, Faculty of Medicine, Cairo University, Egypt

Email

-

City

-

Orcid

-

First Name

Sulayman

Last Name

Fitoury

MiddleName

-

Affiliation

Department of Radiology, Faculty of Medical Technology, Omar Al Mukhtar University, Libya

Email

-

City

-

Orcid

-

First Name

Linda

Last Name

Ibrahim

MiddleName

-

Affiliation

Department of diagnostic and imaging technology, Faculty of Applied Medical Science, 6 October University, Cairo, Egypt

Email

-

City

-

Orcid

-

First Name

M.

Last Name

Morsi

MiddleName

I.

Affiliation

Department of Radiation Science, Medical Research Institute, Alexandria University, Alexandria, Egypt

Email

-

City

-

Orcid

-

Volume

5

Article Issue

3

Related Issue

27855

Issue Date

2021-09-01

Receive Date

2020-12-26

Publish Date

2021-09-01

Page Start

109

Page End

120

Print ISSN

2682-261X

Online ISSN

2682-2628

Article File

JCBR_Volume 5_Issue 3_Pages 109-120.pdf

PDF . 2.6MB

Link

https://jcbr.journals.ekb.eg/article_194075.html

Detail API

https://jcbr.journals.ekb.eg/service?article_code=194075

Order

11

Type

Original Article

Type Code

885

Publication Type

Journal

Publication Title

International Journal of Cancer and Biomedical Research

Publication Link

https://jcbr.journals.ekb.eg/

MainTitle

The diagnostic and predictive values of miRNA-21, miRNA-126, miRNA-513a, and miRNA-98 in patients with non-small cell lung cancer (NSCLC)

Details

Type

Article

Created At

22 Jan 2023