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164757

Evaluation of the anticancer effect of violacein, phycocyanin and phycocyanobilin on apoptotic genes expression and glycan profiles in breast cancer cells

Article

Last updated: 04 Jan 2025

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Tags

Natural/synthetic agents in anti-cancer therapy

Abstract

Background: Cancer researchers have been concentrated on finding the best therapeutic strategies to decline cancer mortality rates. Marine natural products with pharmacological activities have potent anticancer activities. Aim: This study investigated the anticancer effect of violacein, phycocyanin, and phycocyanobilin in MCF-7 cells. Additionally, the elucidation of combined therapy efficiency (violacein-phycocyanin) on enhancing the anticancer activity of monotherapy. Materials and Methods: The cultured untreated, and treated cells by etoposide, violacein, phycocyanin, phycocyanobilin, and combined therapy were subjected to molecular studies, and glycan profiles by real time-PCR, and MALDI-TOF respectively. Results: Bax, Bcl-2, caspase-3 genes expression, and Bax/Bcl-2 ratio in all groups were significantly upregulated compared to the untreated cells except for a significant decrease in Bcl-2 by etoposide or combined therapy and an insignificant difference in Bax/Bcl-2 by PC or PCB treatment. The intensity of tetra-, tri-sialylated N-glycan, and di-sialylated O-glycan was significantly decreased in violacein, and combined-treated cells when compared to untreated or PC-treated cells. Mono-sialylated O-glycan was also decreased in combined therapy compared to the treated or untreated cells. Conclusion: Cotreatment with violacein/PC generated synergistic, antiproliferative, and pro-apoptotic effects on MCF-7 cells. Therefore, these compounds may be promising chemotherapeutic agents for breast cancer

DOI

10.21608/jcbr.2021.46268.1079

Keywords

Apoptotic genes, MALDI-TOF, N-/O-glycans, real time-PCR

Authors

First Name

Neveen

Last Name

Hussein

MiddleName

A.

Affiliation

Applied Medical Chemistry, Medical Research Institute, Alexandria, Egypt.

Email

neveen.hussien@alexu.edu.eg

City

Alexandria

Orcid

0000-0001-9889-8063

First Name

Samia

Last Name

Ebied

MiddleName

-

Affiliation

Medical Research Institute, Alexandria, Egypt.

Email

samia.ebid@alexu.edu.eg

City

Alexandria

Orcid

-

First Name

Marwa

Last Name

Saleh

MiddleName

-

Affiliation

Medical Research Institute, Alexandria, Egypt.

Email

neveen39@yahoo.com

City

Alexandria

Orcid

-

Volume

5

Article Issue

2

Related Issue

24923

Issue Date

2021-06-01

Receive Date

2020-10-13

Publish Date

2021-06-01

Page Start

81

Page End

97

Print ISSN

2682-261X

Online ISSN

2682-2628

Article File

JCBR_Volume 5_Issue 2_Pages 81-97.pdf

PDF . 7.1MB

Link

https://jcbr.journals.ekb.eg/article_164757.html

Detail API

https://jcbr.journals.ekb.eg/service?article_code=164757

Order

8

Type

Original Article

Type Code

885

Publication Type

Journal

Publication Title

International Journal of Cancer and Biomedical Research

Publication Link

https://jcbr.journals.ekb.eg/

MainTitle

Evaluation of the anticancer effect of violacein, phycocyanin and phycocyanobilin on apoptotic genes expression and glycan profiles in breast cancer cells

Details

Type

Article

Created At

22 Jan 2023