Thesis Abstract
Background: Breast cancer is a heterogeneous disease that mandate efficient therapeutics, lower drug toxicity, and overcoming drug resistance. Combination therapy was suggested as a future treatment to attain the required efficacy and tolerable side effects. Aim: The current study aimed to compare and evaluate the efficacy of single and combined treatments in the MCF-7 breast cancer cell line. Materials and Methods: The study included seven MCF-7 groups according to the treatment modality. Cell viability was evaluated by MTT assay at different doses and time course treatments. Results: When nanoparticles were used alone, the starting point of significant cell death was 100 µg, but when using a photothermal combination modality the loss of viability % was bout 2- fold higher than nanoparticles alone especially in low concentrations and there was a significant difference between the 2 groups. There was a significant difference in cell viability between FAC + AuNPs group when compared to FAC only or control group (p ≤ 0.05). There was a significant difference in cell viability between Taxol + AuNPs when compared to control (p ≤ 0.05). there is a significant effect of radiation doses on cell viability within all subgroups ( p < 0.001 for R0, and p=0.001 for R2 subgroup). there is a significant effect of the radiation doses on the cell viability within each subgroup as indicated by significant p values ( p= 0.027 for RN0, and p=0.001 for RN2). Conclusion: Combined GNPs and FAC, paclitaxel, and Radiotherapy treatment modalities could improve breast cancer outcomes and prognosis.