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158660

In vivo and in vitro antitumor effects of Helix desertorum hemolymph by inducing cell cycle arrest and apoptosis

Article

Last updated: 22 Jan 2023

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Tags

Natural/synthetic agents in anti-cancer therapy

Abstract

Background: Natural products, those extracted from invertebrate animals have medical importance as therapeutic agents especially for cancer treatment. Aim of the Work: The current study was conducted to evaluate the antitumor effect of the hemolymph of the desert snail, Helix desertorum (HD-H) on tumor Ehrlich ascetic carcinoma cells (EAC) cell line in vitro and in vivo. Materials and Methods: Helix desertorum (HD-H) was collected from their suitable habitats, identified, and then their hemolymph was collected, to evaluate their antitumor effect. 40 female albino mice were divided into five groups (n=8) as the following: group 1 (Gp1) control mice were inoculated intraperitoneal (i.p) with 1x106 EAC cells/mouseat day 0, Gp2 (EAC/Cis): Mice were injected with EAC-cells as in Gp1 and at day 2 were injected with cisplatin (40 mg/Kg), Gp3 was inoculated with EAC and then treated with splenocytes activated with HD-H, Gp4was inoculated with EAC and then treated with splenocytes activated with IL-2/Con A, and Gp5 was inoculated with EAC and then treated with splenocytes without activation. All groups were sacrificed to evaluate the tumor profile, hematological and biochemical. Results: The results showed that treatment with HD-H led to decrease tumor volume, their cell counts, increase the percentage of the apoptotic cells and arresting the cancer cell cycle. Moreover, treatment with HD-H improved the hematological and biochemical parameters on tumor-bearing mice. Conclusion: In conclusion, HD-H has potential in vitro and in vivo antitumor effects against EAC-cells. Further study is recommended to evaluate the potential efficacy of HD-H as a potential anticancer

DOI

10.21608/jcbr.2021.54156.1105

Keywords

Anticancer, apoptosis, Cell cycle, Helix desertorum (HD-H), Hemolymph

Authors

First Name

Mai

Last Name

Ziada

MiddleName

-

Affiliation

Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt

Email

maiziada5@gmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Mona

MiddleName

-

Affiliation

Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt

Email

monamm3@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Basyony

MiddleName

-

Affiliation

Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt

Email

mohamedbasyony@yahoo.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Salem

MiddleName

-

Affiliation

Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt

Email

mohamedlabibsalem@yahoo.com

City

Tanta

Orcid

-

Volume

5

Article Issue

1

Related Issue

23533

Issue Date

2021-03-01

Receive Date

2020-12-19

Publish Date

2021-03-01

Page Start

155

Page End

164

Print ISSN

2682-261X

Online ISSN

2682-2628

Article File

JCBR_Volume 5_Issue 1_Pages 155-164.pdf

PDF . 3.7MB

Link

https://jcbr.journals.ekb.eg/article_158660.html

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https://jcbr.journals.ekb.eg/service?article_code=158660

Order

15

Type

Original Article

Type Code

885

Publication Type

Journal

Publication Title

International Journal of Cancer and Biomedical Research

Publication Link

https://jcbr.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023