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158655

Role of serum miR-21 and miR-92a in colorectal cancer diagnosis as novel molecular biomarkers

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Last updated: 22 Jan 2023

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Abstract

Background: Our study aimed to evaluate the clinical utility of detecting plasma microRNAs (miR-21 & miR-92a) for diagnosis of colorectal cancer patients and its relation to tumor staging. Patients and Methods: Quantitative real-time RT-PCR was applied to determine the relative expression level of miR-21 and miR-92a in serum. The sensitivity and specificity of these markers were evaluated by receiver operating characteristic (ROC) curve analysis. Final staging of colorectal cancer cases was assigned according to results of histopathologic examination of surgically resected specimens. Results: This study included 52 cases of colorectal cancer (CRC), 20 cases of precancerous colorectal lesions, and 20 healthy controls. Both Plasma miR-21 and Plasma miR-92a were significantly higher in CRC group compared to both the control group and precancerous group. Also, they were significantly higher in advanced CRC stages than early CRC stages. The sensitivity and specificity of miR-21 for discriminating CRC from controls were found to be 90.38% and 100.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 94.23% and 100.0%, respectively. For discriminating CRC cases from precancerous lesions, the sensitivity and specificity of miR-21 were found to be 75.08% and 95.0%, respectively. However, for miR-92a, sensitivity and specificity were found to be 80.77% and 100.0%, respectively. Conclusions: both plasma miR-21 and miR-92a have significant value for early detection of CRC as non-invasive screening molecular biomarkers with high sensitivity and specificity. They also help for differentiation between patients with benign and malignant colorectal lesions and those with early and advanced CRC.

DOI

10.21608/jcbr.2020.34838.1049

Keywords

biomarkers, colorectal cancer, microRNAs, miR-21, miR-92a

Authors

First Name

Ramy

Last Name

Hassan

MiddleName

-

Affiliation

General Surgery Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

ramyhassan@aun.edu.eg

City

-

Orcid

-

First Name

Mohamed

Last Name

Omar

MiddleName

-

Affiliation

Department of surgical oncology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt

Email

mo7esomar@gmail.com

City

Asyut

Orcid

0000-0003-0921-7700

First Name

Mahmoud

Last Name

Shehata

MiddleName

-

Affiliation

General Surgery Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

drmahmoudrefaat@yahoo.com

City

-

Orcid

-

First Name

Mohamad

Last Name

Raafat

MiddleName

-

Affiliation

General Surgery Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

mohamad_raafat10@yahoo.com

City

-

Orcid

-

First Name

Ali

Last Name

Hamdy

MiddleName

-

Affiliation

Clinical Pathology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

alihamdy@aun.edu.eg

City

-

Orcid

-

First Name

Ali

Last Name

Zedan

MiddleName

-

Affiliation

Department of surgical oncology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt

Email

alizedan73@yahoo.com

City

-

Orcid

-

First Name

Murad

Last Name

Jabir

MiddleName

-

Affiliation

Department of surgical oncology, South Egypt Cancer Institute, Assiut University, Assiut, Egypt

Email

muradjabir@aun.edu.eg

City

-

Orcid

-

Volume

5

Article Issue

1

Related Issue

23533

Issue Date

2021-03-01

Receive Date

2020-07-05

Publish Date

2021-03-01

Page Start

95

Page End

104

Print ISSN

2682-261X

Online ISSN

2682-2628

Article File

JCBR_Volume 5_Issue 1_Pages 95-104.pdf

PDF . 2.8MB

Link

https://jcbr.journals.ekb.eg/article_158655.html

Detail API

https://jcbr.journals.ekb.eg/service?article_code=158655

Order

9

Type

Original Article

Type Code

885

Publication Type

Journal

Publication Title

International Journal of Cancer and Biomedical Research

Publication Link

https://jcbr.journals.ekb.eg/

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Article

Created At

22 Jan 2023