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105567

Evaluation of the anti-Fusarium effect of Cinnamoum zeilanicum, Berberise vulgaris and Caluna vulgaris ethanolic extracts

Article

Last updated: 22 Jan 2023

Subjects

-

Tags

Cellular and molecular targeting

Abstract

Background: Mycotoxins such as Fumonisin B (FB), are low-molecular-weight natural products produced as secondary metabolites by filamentous fungi. The mycotoxins, FB, contaminates grain products and acts as a virulence factor for host cells. Aim: This study aimed to evaluate the effect of ethanolic crude extracts of Cinnamuom zeilanicum (CZ), Berberise vulgaris (BV) and Calluna vulgaris (CV) on the growth of corn seed's fungi and /or mycotoxin production. Materials and methods:  Firstly, corn seeds were collected and cultured on water agar media and the grown fungi were inoculated into Sabouraud's dextrose media, then the toxins were identified. Secondly, the dried plants of CZ, BV and CV were phytochemically screened for alkaloids, phlobatannins, saponnins, flavonoids, steroids, terpenoids and cardiac glycosides. The fungus purified DNA was sequenced, and BLAST alignment was used to identify the fungi and mycotoxin types. Furthermore, the effect of different plant extract treatments on fungi growth was assessed. Finally, toxinproduction was assessed using HPLC. Results: The obtained results confirmed that Fusarium isolate was Fusarium sporotrichioides. Among the three tested extracts, Cinnamuom zeilanicum extract was the most potent inhibitor for Fumonisin B production followed by Berberise vulgaris and Calluna vulgaris. Conclusion: Cinnamuom zeilanicum extract could be used during corn seed long-term packaging and storage to inhibit (FB) production and toxicity.

DOI

10.21608/jcbr.2020.30493.1039

Keywords

Corn seeds, fumonisin B1, Fusarium sporotrichioides, Mycotoxins, zearalenone

Authors

First Name

Nadia

Last Name

Ismail

MiddleName

-

Affiliation

Medical laboratory technology Department, Faculty of Allied Medical Sciences, Pharos University in Alexandria, Alexandria, Egypt.

Email

nadia.elyas@pua.edu.eg

City

-

Orcid

-

First Name

Doaa

Last Name

Ghareeb

MiddleName

-

Affiliation

Department of Biochemistry, Faculty of Science, Alexandria University, Egypt

Email

d.ghareeb@yahoo.com

City

-

Orcid

-

First Name

Sobhy

Last Name

El- Sohaimy

MiddleName

-

Affiliation

Department of Food Technology, City for Scientific Research & Technology Applications, New Borg El Arab, Alexandria, Egypt.

Email

selsohaimy@srtacity.sci.eg

City

-

Orcid

-

First Name

Maha

Last Name

EL-Demellawy

MiddleName

-

Affiliation

Department of Medical Biotechnology, Genetic Engineering & amp; Biotechnology Research Institute, City for Scientific Research & Technology Applications, New Borg El Arab, Alexandria, Egypt.

Email

m.eldemellawy@mucsat.sci.eg

City

-

Orcid

-

First Name

Mohamad

Last Name

El-Saied

MiddleName

-

Affiliation

Department of Biochemistry, Faculty of Science, Alexandria University, Alexandria Egypt.

Email

mmaelsayed1@yahoo.com

City

-

Orcid

-

Volume

4

Article Issue

2

Related Issue

12003

Issue Date

2020-08-01

Receive Date

2020-05-17

Publish Date

2020-08-01

Page Start

143

Page End

150

Print ISSN

2682-261X

Online ISSN

2682-2628

Link

https://jcbr.journals.ekb.eg/article_105567.html

Detail API

https://jcbr.journals.ekb.eg/service?article_code=105567

Order

8

Type

Original Article

Type Code

885

Publication Type

Journal

Publication Title

International Journal of Cancer and Biomedical Research

Publication Link

https://jcbr.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023