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93035

High expression of the checkpoint molecule PD-1 on regulatory and helper CD4+ T cells in metastatic breast cancer patients with poor prognosis

Article

Last updated: 22 Jan 2023

OverviewSimilar Items

Subjects

-

Tags

Anti-cancer Immunotherapy
Cell-based therapy against cancer
Cellular and molecular targeting

Abstract

Background: Recurrence and metastasis are common in patients of breast cancer even after chemotherapy and radiotherapy. This recurrence may occur due to the emergence of immunoregulatory T (Treg) cells and the immune checkpoint molecules such as programmed cell death protein 1 (PD-1) which are known to suppress anti-tumor immunity. Aim: The aim of this study is to investigate the numbers of Treg cells and the expression of PD-1 molecule on T cells in the peripheral blood of breast cancer patients at different stages (II, III, and IV) compared to healthy individuals. Materials and Methods: Blood samples were collected from early diagnosed metastatic cancer patients before surgery. Leucocytes were stained with different antibodies including CD4, CD25, CD127, and PD1, and then were analyzed by multiparametric flow cytometry. Results: The results showed that the relative and absolute numbers of Treg cells were significantly increased in breast cancer patients. Interestingly, we found a correlation between the numbers and cancer progression from stages II to III and IV, as compared with healthy individuals. Furthermore, significant increases in PD-1 expression were observed on Treg cells and CD4+ T cells in cancer patients as compared to healthy controls. This profile of PD-1 expression was also correlated with the poor prognosis of the patients. Conclusion: The results provide a better understanding of the immunomodulatory role of Treg cells and PD-1 expression in breast cancer patients who are the target for immunotherapy using checkpoint inhibitors. 

DOI

10.21608/jcbr.2020.22585.1010

Keywords

Breast Cancer, CD4+ T cells, T regulatory cells, checkpoint molecules, programmed cell death protein, PD-1, Flow cytometry

Authors

First Name

Mohamed

Last Name

Esmail

MiddleName

Ahmed

Affiliation

Zoology Department, Faculty of science, Tanta university, Tanta, Egypt

Email

mohamed.esmail820@yahoo.com

City

Mansoura

Orcid

-

First Name

Mohamed

Last Name

Attia

MiddleName

-

Affiliation

Department of Clinical Pathology, Faculty of Medicine, Tanta University, Tanta, Egypt.

Email

attia17@yahoo.co.uk

City

-

Orcid

-

First Name

Sameh

Last Name

Abd-Alaziz

MiddleName

R

Affiliation

Department of Oncology Surgery, Oncology Center, Mansoura University, Mansoura, Egypt

Email

-

City

Mansoura

Orcid

-

First Name

Sabry

Last Name

El-Naggar

MiddleName

-

Affiliation

Zoology Department, Faculty of Science, Tanta University, Tanta, Egypt

Email

sabry_elnaggar@yahoo.com

City

-

Orcid

0000-0002-7018-9350

First Name

Mohamed

Last Name

Salem

MiddleName

Labib

Affiliation

Immunology and Biotechnology Division, Department of Zoology, Faculty of Science, Tanta University, Egypt

Email

mohamed.labib@science.tanta.edu.eg

City

Egypt

Orcid

0000-0001-9454-6327

Volume

4

Article Issue

1

Related Issue

10127

Issue Date

2020-04-01

Receive Date

2020-01-17

Publish Date

2020-04-01

Page Start

69

Page End

77

Print ISSN

2682-261X

Online ISSN

2682-2628

Article File

JCBR_Volume 4_Issue 1_Pages 69-77.pdf

PDF . 1.8MB

Link

https://jcbr.journals.ekb.eg/article_93035.html

Detail API

https://jcbr.journals.ekb.eg/service?article_code=93035

Order

9

Type

Original Article

Type Code

885

Publication Type

Journal

Publication Title

International Journal of Cancer and Biomedical Research

Publication Link

https://jcbr.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

22 Jan 2023