Background: T cells are crucial for the inflammation development of the metabolic disorder and insulin resistance and the cytokines of T cells are considered as an excellent evaluating tool for the immune system activity in many diseases. As cytokine profile associates with the development of type-2 diabetes mellitus (T2-DM) is well understood the link between this disease and the involved immune cells still unclear. as such, it was hypothesized that the Th1 pathway is disrupted in the pathogenesis of type 2DM. Aim: The main aim of this study was to evaluate IL-12, which induces the Th1 pathway, and IL-2 which mediates the Th1 function. Materials and Methods: Paraffin-embedded sections of peripheral blood lymphocytes with T2-DM patients were stained with antibodies against CD4⁺ and CD8⁺ T cells. Computerized image analysis was used to calculate areas of stained lymphocytes taken from diabetic and control patients. Clinical data and blood samples were collected from Eighty-one (37.6±13.9 years old) diabetic Egyptian patients and 103 (40.5±12.7 years old) of normal control volunteer to evaluate the level of serum interleukin IL-2 and IL-12 in their blood by using ELISA technique. Routine laboratory analysis, including fasting blood glucose, CRP, lipid profile, fasting insulin, and CBC for each patient were measured. Results: Immunohistochmical results in 2DM showed an increase in the numbers of CD8⁺ cells with a decrease in the numbers of CD4⁺ cells displayed. Diabetic patients showed decreased and increased values of IL-2 and IL-12, respectively as compared to control values. Conclusion: This data indicates the importance of these two cytokines in the pathogenesis of type-2 Diabetes mellitus.