Background: Carcinogens become a serious environmental challenge. Aim: The main aim of this study was to assess the alterations in some biochemical parameters in male Wistar rats after. Materials and Methods: Experimental groups were administered with 0.05% N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) followed by 5% Sodium ascorbate (Na-As), then they were post-treated with Nigella sativa (N. sativa) by an inter-gastric luminal gavage or with thymoquinone (TQ) by inter-peritoneal injections. Blood samples were collected after a 32 weeks' experiment. Results: TQ seemed to have a powerful inhibitory effect on total cholesterol and triacylglycerol levels. While levels of A/G ratio, ALT and AST were highly decreased by both of N. sativa and TQ. Conclusion: These findings may rely on the lipid-modifying and hepatoprotective effects of N. sativa crude oil and its main constituent TQ on mammals. Thus, further investigations are required.