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252693

CITRIC ACID PRODUCTION BY Aspergillus niger REPEAT· BATCH CULTURE USING A . PRICKLY PEAR CACTUS EXTRACT MEDIUM

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Last updated: 04 Jan 2025

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Abstract

The potential of prickly pear cactus extract as a fermentation medium for
citric acid production by batch and repeat-batch cultures of Asp < /em>ergillus niger isolated
from three locations of Aseer, Saudi Arabia was investigated. Among the 23 isolated
cultures of Aspergillus niger, the isolate J6 which obtained from Jazan was found to
be enhance citric acid production. Maximum citric acid production (61.56 g/L)was
obtained after 8 days of cultivation with an initial pH 4 and 20 % sugar concentration.
Better results were achieved when applying repeat-batch culture than batch
cultivation. The culture was retained their activity for 24 days of fermentation period
and the concentration of citric acid was remained constant during recycle of biomass
up to the third batch. A total amount of citric acid of 244.75 g was obtained after five
repeated batch-cultivation. On the basis of the experimental results, it was suggested
that the prickly pear cactus extract can serve as a low-cost fermentation medium for
citric acid production using Aspergillus nigar repeat batch-culture.

DOI

10.21608/jacb.2003.252693

Authors

First Name

A.

Last Name

Al-Shehri,

MiddleName

M.

Affiliation

King Khalid University. College of Science. Biological Science Dept. Abha P.O.Box 9004. Saudi Arabia

Email

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City

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Orcid

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First Name

Y.

Last Name

Mostafa

MiddleName

S.

Affiliation

King Khalid University. College of Science. Biological Science Dept. Abha P.O.Box 9004. Saudi Arabia

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Volume

28

Article Issue

7

Related Issue

35974

Issue Date

2003-07-01

Receive Date

2003-06-05

Publish Date

2003-07-01

Page Start

5,653

Page End

5,660

Print ISSN

2090-3626

Online ISSN

2090-3707

Article File

JACB_Volume 28_Issue 7_Pages 5653-5660.pdf

PDF . 1.1MB

Link

https://jacb.journals.ekb.eg/article_252693.html

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https://jacb.journals.ekb.eg/service?article_code=252693

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Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

CITRIC ACID PRODUCTION BY Aspergillus niger REPEAT· BATCH CULTURE USING A . PRICKLY PEAR CACTUS EXTRACT MEDIUM

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Article

Created At

22 Jan 2023