This study was carried out to investigate the effect of several agro-industrial wastes and mixed substrates with rice straw (w/w) on production of ( Pleurotus ostreatus ). Mixed substrates with rice straw at rate 1:1 by weight showed higher effect on the first stage, second and three stages and data indicated  that the time for production ranged from 12 - 25.5, from 22 - 52.25 and from 25 - 55 days respectively. When mixed substrates with rice straw the results were found within the period ranged from 11.75 - 18.5, from 22.5 -28.5 and from 26.5 - 32 days respectively. Mixed substrates with rice straw reduce the first stage time of oyster compare with the control substrates. Therefore reduce pinheads and fruiting bodies formation period. The mixed substrates rice straw with waste paper (RS+WP) gave the maximum of oyster mushroom produced per 1 kg dry ~ 578.50 g and gave the maximum biological efficiency 57.85 %. Finally, mixed substrates with rice straw increase in biological efficiency of P. ostreatus over control substrates.