Syzygium cumini belongs to the family L. to Myrtaceae which are consumed as cherry-shaped fruits and are called pamposa in Egypt. The extracts of this fruit possess effective substances that haveantimicrobial, antioxidant and anti-cancer effects. The methanolic extracts of S. cumini fruit and seeds were tested on three types of human cancer cells, and also as anti-microbial substances on six microorganisms, three bacteria and three fungal strains. The methanolic extract of unripe fruit pulp has the strongest inhibition (82.2%) against DPPH radical using 25 μg/mL while methanolic extract of ripe fruits pulp gave lowest inhibition value of 28.02% at the same concentration. The results showed that the methanolic extract (0.1%) of the pulp of the ripe fruits was more effective on microbial strains and fungal strains. The methanolic extract of unripe fruit seeds of S. cumini was showed that the best anticancer activity was obtained using the high concentration of the tested samples (1000 μg/ml) against colorectal adenocarcinoma (Caco2) with an IC50 value was 30.93.