Six pullulan producing strains of Aureobasidium pullulans were obtained and
tested (or pull ulan production on three different media. All aureobasidiurn tested
strains gave the maximum pullulan production on Reeselev & Jensen medium. Only one strain was selected as highly efficient pullulan producing fungus known as Aureobasidium pullufans ATCC 42023. The effect of some nutritional and
environmental factors on pullulan production by this strain on Reeselev & Jensen
medium were evaluated using shake flask as a batch culture. A. pullulans ATCC
42023 gave the highest pullulan production parameters after 5 days fermentation
period at 2SoC in modified Reeselev & Jensen medium containing 5 % sucrose and 0.05 % glutamic acid (as a carbon and nitrogen sources, respectively) and the ratio of flask size to working volume was 2.5 ; 1. Using HCDI two-stage batch culture for pullulan production on modified Reeselev & Jensen medium (20 % sucrose) led to increase cell dry weight and puUulan productivity by 2.28 and 2.65 fold than that obtained in batch shake flasks technique.