The study carried out investigate the effect of different agro-industrial wastes and mixed substrates with rice straw (w/w) on caltivation of oyster mushroom ( Pleurotus florida ). Mixed substrates with rice straw at rate 1:1 by weight had valuable effect on the spawn running, duration pinhead's formation and fruiting bodies formation. Results regarding to the time required for completion were found within the period ranged from 11.25 - 27, from 23.5 - 51 and from 27 - 54 days respectively. When mixed substrates with rice straw the spawn running, pinheads and fruiting bodies formation were found within the period ranged from 10.5 - 19.25, from 22 - 26.5 and from 27 - 32 days respectively. Mixed substrates with rice straw reduce the spawn running period of P. florida over control substrates. Therefore, reduce pinheads and fruiting bodies formation period. The highest total weight of mushroom harvested per 1 kg dry mixed substrate was recorded on rice straw with waste paper (RS + WP) ~ 853.87 g and gave the maximum biological efficiency 85.49 %. Mixed substrates with rice straw increase in biological efficiency of P. florida over control substrates.