Prickly pear seed oil represents 10% of dry seed. and could be considered one ‘of the important nutritional oils. The procedure of gas liquid chromatography (GLC) possessed five fatty acids based on outhentic samples. linoleic acid was the dominant monounsaturated fatty acid, it representing 68.27% of total fatty acids. Concerning rats feeding, the analysis of blood serum declared that the addition of prickly pear seed oil and defatted seed fiber to the rich fat diet reduced cholesterol. LDL cholesterol and triglycerides. High percent of vitamin E was detected in rats serum fed on 0.5ml and 0.25mi from seed oil compared to the positive control rats fed on hyperiipidemic diet . They were 27.15 :1: 2.48 mg I100ml. 24.41 :3.54 mgl100 ml. and 15.89 a: 0.60 mg! 100ml. respectively. Statistical analysis revealed significant differences between groups concerning vitamin E and Iipidperon‘dation (MBA). Histopathological examinations of experimental rats liver were performed and observed. The examined liver of rats fed on diet supplemented with seed oil, showed a distinect characterization like that of rats fed on basal diet (negative control rats). which showed the normal histology of hepatic lobule. From the obtained data, it can be seen that the prickly pear seed oil could be considered one of the greatest dietary seed oil which would be used to protect from several diseases caused by free radicals accumulation.