In this study. a greenhouse pot experiment was designed to investigate the response of Artemisia plants to fertilization with different nine treatments as a combination of bibfertillzers (Azoiobacler + Azospirifiivn + phosphate dissolving bacteria) with different forms of organic and/or inorganic fertilizers in two different soil types (sandy soil from Wadi El-Natrun and calcareous soil from Ei-Khatataba). Which are commonly found in the Egyptian desert soils. Obtained data showed that the tested Artemisia plants significantly responded to biofertilization treatment, which was very clear in the enhancing the values of both fresh and dry weights of roots and shoots of this herb. total nitrogen and phosphorous content. and its quality as indicated by the percentage of the main active substances (cineol and myrecen) characteristic of this medicinal plant. Again. the antimicrobial activities of artemisia essential oil against some tested organisms [G- and 6+ bacteria as well as fungi and yeast] were positively affected due to bio- fertilizatlon and/or organic manuring. The best measurements were obtained by using the treatment no. 8. which included the application of Azofobecier + Azospiriiium + phosphate dissolving bacteria + full dose of K + quarter-dose of N + rock phosphate + sheep dung manure. These results support the emphatic idea for using blofertilization for reducing the costs of mineral fertilization and the problems of environmental pollution.