It is will know that drinking water is very important and essential for human life. If water is not good or polluted, human health will be affected and it will cause many diseases. This study was conducted to evaluate the quality (bacteriological and chemical) of the drinking water  which collected from tab water , ground water , River Nile water and bottled water from different cities in El-Dakahlia and Damietta Governorates , Egypt during four season , summer , autumn , winter and spring . Five bacterial isolates (Escherichia coli, Kleibsila pneumoniae, Staphylococcus aureus, Staphylococcus epidermidis and Streptococcus sp.  were choosed and identified by morphologically, biochemically and molecularly as well as tested for antibiotics sensitivity. Results showed that there are difference between bacterial isolates sensitivity and different groups of antibiotics. Also, the water samples were physically and chemically analyzed for temperature, pH, electrical conductivity(EC), turbidity, total dissolved solids (TDS), total alkalinity, total hardness, ammonia, nitrate, nitrite, chloride, sulfate, phosphate, calcium hardness and magnesium hardness. High values as shown of Damietta for turbidity, pH, total alkalinity, total hardness and sulfate in tab water. In ground water, most significant high values of TDS in all water sources in El-Dakahlia . On other hand, the total alkalinity and total hardness of Damietta River Nile water recorded highest values compare with El-Dakahlia Governorate. In bottled water, the lowest values were observed compared with other sources of water especially in physical properties for water samples.