A pot experiment was carried out at the experimental greenhouse of the Faculty of Agriculture, Mnsoura University, Dakahlia, Governorate, to study the effect of Azotobacter and/or cyanobacteria inoculation each individually and/or both in combinations under three nitrogen levels (zero N, 1/2 full N recommended dose and full N recommended dose) on wheat (Triticum aestivum L.) variety Sakha 93. Some soil biological, physical and chemical properties were also studied. Results indicated that the dual inoculation with both cyanobacteria and Azotobacter, generally enhanced wheat plant growth and increased wheat grain and straw yields, NPK uptake by grains and straw, available NPK in soil at three stages of wheat growth (vegetation, panicle initiation and at harvest). As well as the soil biological activity was positively enhanced due to the dual inoculation with both cyanobacteria and Azotobacter  combined with 1/2 N dose only especially at the second stage (panicle initiation). In this concern, this treatment led to increase the soil dehydrogenase activity, CO₂ evolution, soil microbial community represented by total bacteria count, total cyanobacteria count and Azotobacter count. However, the priority was for the second stage (panicle initiation) and the treatment of ½ N + cyano + Azoto compared to the other tested treatments and/or stages.   In conclusion, much attention should be paid to understand the mechanism of dual inoculation with both cyanobacteria and Azotobacter that positively affected wheat production and improved biological and chemical characters for the inoculated soil.