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In present study the capability of some microorganisms isolated from Egyptian soil to a triazine herbicide, atriazine, decomposition was assessed. Nine atrazine-degrading bacteria were isolated from soil that received repeated exposures of the commonly used herbicides atrazine. These isolates were belonged to genera Basilllus, Pseudomonas and Micrococcus. One isolate showed good growth and clearing zone on mineral salts agar media amended with atrazine (at 500 ppm) as a carbon and/or nitrogen source. This most active strain involved in atrazine degradation were selected and identified. The strain was classified as Bacillus megaterium. It degraded 45.8% of initial concentration of atrazine concurrent with increasing the population size from 105 to 108 CFU/ml culture. Atrazine-degrading enzymes by B. megaterium appear to be induced. No released chloride was detected from B. megaterium culture indicating that the triazine ring may be remained intact and the atrazine-metabolites not hydroxyatrazine. Atrazine metabolites may be deethylatrazine or deisopropylatrazine. These results indicate that B. megaterium can play an important role in the bioremediation of atrazine-contaminated sites.
DOI
10.21608/jacb.2008.200726
Keywords
atrazine, Basilllus, Pseudomonas and Micrococcus, Degradation, Bacillus megaterium, bioremediation
Authors
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Microbiol. Dept., Fac. Agric., Mansoura Univ., Mansoura, Egypt.
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Microbiol. Dept., Fac. Agric., Mansoura Univ., Mansoura, Egypt.
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Microbiol. Dept., Fac. Agric., Mansoura Univ., Mansoura, Egypt.
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Soil, Water and Environ. Res. Instit., Agric. Res. Center (ARC), Giza.
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-Orcid
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Microbiol. Dept., Fac. Agric., Mansoura Univ., Mansoura, Egypt.
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https://jacb.journals.ekb.eg/article_200726.html
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https://jacb.journals.ekb.eg/service?article_code=200726
Publication Title
Journal of Agricultural Chemistry and Biotechnology
Publication Link
https://jacb.journals.ekb.eg/
MainTitle
ATRAZINE DEGRADATION BY Bacillus megaterium ISOLATED FROM AGRICULTURAL SOIL