This research was conducted for two consecutive seasons (2017 and 2018) in a private vineyard located at El-Sadat city, Menofia governorate to describe and evaluate three grape seedless cultivars namely: Early Sweet, Prime and Star light grape cultivars under Egyptian conditions. The results revealed that Early Sweet grape cultivar was the earliest cultivars concerning the phenological dates include bud burst, full bloom, fruit set, veraison and physiological ripening followed by Prime grape cultivar, whereas Star light grape cultivar was the latest in this respect through the two seasons of the study. All the studied cultivars succeeded under Egyptian conditions and characterized by high quality of bunch and berries. Genetic diversity among the three cultivars was assessed using SCoT and ISSR molecular marker techniques. Cluster analysis showed molecular and phenotypic variation among cultivars, which divided into three groups, each group was contained one cultivar. These cultivars were distinguished by 36 molecular markers 27 out of them were from SCoT markers which proved successful to target generic regions across the grape genome. These markers after deciphering their structure may help to evolve reliable molecular markers to select desirable traits in these cultivars. So, we recommend expansion in the cultivation of these cultivars under Egyptian conditions and benefit of our molecular results to elicit reliable molecular markers characterizing desirable traits in these cultivars to use in Egyptian grape cultivars improving programs.