Cymbopogon citratus, universally known as Lemongrass is a small herbaceous plant of Poaceae family. It is used as traditional medicine for treatment of numerous diseases such as fever, sore throats, cough, laryngitis, bronchitis, oral candiasis, body ache, head ache, digestive problems etc. This study deals with the antioxidant activities such as DPPH and FRAP of mehanol extract of lemon grass leaves followed by quantitative analysis of total phenolics (TPCs) and flavonoids (TFCs) content. Antimicrobial activity of the extract was also been tested to highlight the medicinal values. The methanol extract showed significant antioxidant activity using DPPH and FRAP (26.03±1.60 μM; 922.43 μM trolox/100 g dry weight, respectively) compare to the standard Trolox.C. citratus extract has phenolic and flavonoid content as 130.33±1.23; 193.63±4.63, respectively. The results showed that extract of C. citratus exhibited maximum zone of inhibition (35mm) against Bacillus subtilis. at the highest concentration 150mg/ml methanol extract.