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148054

Evaluation of some Tomato Genotypes for Nematode Resistance and Detection of the Mi-1.2 Resistance Gene

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Last updated: 04 Jan 2025

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Abstract

Response of fourteen tomato genotypesfrom different geographical regions to root-knot nematodes (RKN; Meloidogyne spp < /em>.) was evaluated for two seasons under greenhouse conditions. Two PCR-based markers (Mi-23 and REX-1) were used for the detection of the RKN resistance gene (Mi-1.2) in the tested genotypes. The results showed a wide variation among tested tomato genotypes in their responses to RKNs. Highly significant differences were observed among tested genotypes for gall index and egg masses per root system. Based on nematode bioassay, the tomato genotype Strain B F1 was highly resistant to RKN and ranked in top of resistant genotypes. Floradade and VF145-B were resistant, Castlerock, Peto86 and Qaha were moderately resistant, Super strain B, E115, Super Marmande, M82 and Edkawy were susceptible, and E37, E40 and Giza-80 were highly susceptible to RKN. Molecular analysis with Mi23 and REX-1 markers indicated a heterozygous state (Mi/mi) of Mi-1.2 gene for Strain B F1; meanwhile, the remaining genotypes displayed a homozygous recessive state (mi/mi). These finding suggested that Strain B F1 could be used to develop new tomato cultivars resistant to RKN through tomato breeding programs. In addition, the results suggested that tomato genotypes that were identified in the present study as homozygous recessive (mi/mi) for the Mi-1.2 gene and considered as resistant or moderately resistant may possess other genes controlling RKN resistance. However, further studies are still needed to determine these genes that control the resistance to RKNs in tomatoes.

DOI

10.21608/jacb.2021.54585.1008

Keywords

Root-knot nematode (RKN), Meloidogyne spp, Solanum lycopersicum, Mi-1.2 gene, Mi-23 and REX-1 markers

Authors

First Name

Mohamed

Last Name

Hassan

MiddleName

I.

Affiliation

Department of Genetics, Faculty of Agriculture, Assiut University, Egypt

Email

m_hassan79@aun.edu.eg

City

-

Orcid

0000-0001-6608-2369

First Name

A.

Last Name

Mahmoud

MiddleName

F.

Affiliation

Department of Plant Pathology, Faculty of Agriculture, Assiut University, Assiut, Egypt.

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Orcid

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Volume

12

Article Issue

1

Related Issue

20355

Issue Date

2021-01-01

Receive Date

2021-02-12

Publish Date

2021-01-01

Page Start

5

Page End

10

Print ISSN

2090-3626

Online ISSN

2090-3707

Article File

JACB_Volume 12_Issue 1_Pages 5-10.pdf

PDF . 711.8kB

Link

https://jacb.journals.ekb.eg/article_148054.html

Detail API

https://jacb.journals.ekb.eg/service?article_code=148054

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2

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Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

Evaluation of some Tomato Genotypes for Nematode Resistance and Detection of the Mi-1.2 Resistance Gene

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Article

Created At

22 Jan 2023