The up-to-date technique of start codon targeted (SCoT) polymorphism (sites around start codon) is efficient in genetic diversity analysis and fingerprinting. In the current study, 94 amplified fragments were obtained using 10 SCoT Primers with 10 genotypes of Cicer arietinum L with 35% polymorphism, where the polymorphic bands were 33 and 61 bands were monomorphic. The SCoT data analysis showed genetic similarity ranging from 84 to 98%. A dendrogram separated the studied ten chickpea genotypes into two major clusters, divided into two main sub-clusters. The difference in Principal Coordinate Analysis (PCA) established genetic variance between the investigated samples. In four quadrants, the PCA variable demonstrated clustering for the 10 genotypes of chickpea that were examined.