Yield performance and berry sensory attributes in 10 grape cultivars were studied under Assiut conditions. In addition, the genetic variability and relationships among these cultivars were determined based on RAPD and SRAP molecular markers. Genetic variations were observed between the tested cultivars in all studied traits of yield and yield components as well as in berry sensory attributes. 100 sites of grape genome were amplified by RAPD and SRAP primers and used to study the interrelationships between 10 grape cultivars. The highest interrelationships were found between “Black Muscat and Provano", “Palomino and Bez El-Naka", and between “Palomino and Ruby Seedless". The lowest relatedness was found between Beauty Seedless and the other nine grape cultivars. The phenotypic data and molecular markers were effective in estimating the genetic variability between grape cultivars. The study indicated the presence of abundant genetic variability among some of the important commercial grape cultivars. Significant positive correlation was found between the phenotypic and genotypic distance indicated that the studied RAPD and SRAP markers were able to bind to effective regions in the genome.