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137305

Genetic Relationships and Diversity among Pea (Pisum sativum L.) Genotypes Assessed using Agro-Morphological and Molecular Markers

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Last updated: 24 Dec 2024

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Abstract

Fifteen pea (Pisum sativum L.) genotypes were evaluated for their agro-morphological performance and molecular diversity during 2017-2018 and 2018-2019 seasons. Combined analysis of variance revealed that there are highly significant differences among pea genotypes tested for all agro-morphological traits studied. The phenotypic coefficients of variation (PCV) values were relatively greater than Genotypic coefficients of variation (GCV) for all traits; however, GCV values were near to PCV values for the traits, like plant height, pods/plant, 100 seeds weight and pod width. High heritability coupled with high genetic advance was obtained for, plant height, reflecting the presence of additive gene action for the expression of this trait. High to moderate heritability coupled with moderate genetic gain were exhibited for number of pods per plant, 100 seeds weight, pod length, seeds per pod, pod weight and number of branches per plant. The Euclidean Distance among all genotypes based on agro-morphological traits was relatively wide. ISSR and SRAP markers were used to study the molecular diversity among pea genotypes. The molecular marker parameters revealed that SRAP markers were more efficient than ISSR markers with regards to polymorphism detection and in distinction among pea genotypes. Also, both ISSR and SRAP markers were able to amplify unique bands specific to a particular genotype.  A positive and significant correlation (r=0.389: p ≤ 0.001) between ISSR and SRAP matrix was observed according to Mantel's test. Also, significant correlation (r = 0.411: p ≤ 0.001) between the matrices of combined molecular markers data and agro-morphological data.

DOI

10.21608/jacb.2020.137305

Keywords

pea, variability, heritability, genetic advance, ISSR, SRAP

Authors

First Name

B.

Last Name

Abd El-Fatah

MiddleName

E.S.

Affiliation

Department of Genetics, the Faculty of Agriculture, Assiut University Assiut 71526, Egypt

Email

bahaaeldeen.eisa@agr.au.edu.eg

City

-

Orcid

-

First Name

Dina

Last Name

Nafea

MiddleName

S.

Affiliation

Department of Vegetables, the Faculty of Agriculture, Assiut University Assiut 71526, Egypt

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Volume

11

Article Issue

12

Related Issue

20342

Issue Date

2020-12-01

Receive Date

2021-01-09

Publish Date

2020-12-01

Page Start

353

Page End

363

Print ISSN

2090-3626

Online ISSN

2090-3707

Link

https://jacb.journals.ekb.eg/article_137305.html

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https://jacb.journals.ekb.eg/service?article_code=137305

Order

2

Type

Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

Genetic Relationships and Diversity among Pea (Pisum sativum L.) Genotypes Assessed using Agro-Morphological and Molecular Markers

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Article

Created At

22 Jan 2023