Jatropha curcas L. (Jatropha) is a perennial plant belongs to family Euphorbiaceae and well-known for its great capacity of producing latex exudates from the stem and high content of seed oil used in biodiesel production. J. curcas different extracts and exudates are possess many pharmacological effects as anticancer, hepatoprotective and wound healing. The present study aimed to evaluate the different extracts of Jatropha curcas as anti-cancer activity by in vitro against MCF-7, HepG2 and HCT-116 cancer cell lines. The highest in vitro cytotoxic activity of curcin and methanolic extract of Latex were towards HepG2 cell line (IC₅₀ 36.7, and 19.11 μg/ml respectively). In addition, in vivo antitumor activity against chemically induced rat hepatocellular carcinoma (HCC) model showed that curcin showed marked ameliorations in all evaluated liver functions.Moreover, histopathological assessments as well as the tissue levels of certain oxidative stress biomarkers strongly assured the beneficial effects of curcin in fighting DENA-induced HCC.