Establishment of an efficient adventitious shoot regeneration system for mature embryo of Prunus persica via shoot organogenesis has been achieved. Three different combinations of plant growth regulators (PGRs) have been applied in the current study; 6-Benzyl adenine (BA) or thidiazuron (TDZ) with Indole acetic acid (IAA) or Indole butyric acid (IBA). The high regeneration frequency was illustrated by 1.5 mg/l BA with 0.5 mg/l IAA combinations. Quantitative expression of eight epigenetic regulatory genes; Chromomethylase 3 (CMT3), Domains Rearranged Methyltransferase2 (DRM2), Fertilization Independent Endosperm (FIE2) and Chromodomain Protein Like Heterochromatin Protein1 (LHP1), Pickle (PKL), Proliferating Cell Nuclear Antigen (PCNA), Shoot Meristemless (STM) and DICER-LIKE1 (DCL1) have been estimated in the peach regenerated shoots. We found that the auxins and cytokinins in different combinations especially BA effect on the expression of some epigenetic genes in relation to the mature embryo.