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Using Clover Husk for Avicelase Production from Aspergillus flavus Via Solid-State Fermetnation

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Last updated: 04 Jan 2025

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Abstract

Avicelase is considered as an important component in the cellulases system, which has great importance in the saccharification of cellulosic materials for use in biotechnological industries. Recently, there is a trend to produce cellulolytic enzymes from microorganisms especially fungi through solid-state fermentation. In this study, a high avicelase producing local fungus was isolated and identified as Aspergillus flavus isolate L-2007/2012, then used for high avicelase production. Among some cellulosic wastes, clover husk was chosen as the best basal fermentation substrate. Results of avicelase production showed that the highest production of avicelase 799.23 U/g was obtained after 7 days of incubation by adjusting moisture ratio at 1.5:1.0 (v/w) and supplementing fermentation medium with 1% glucose as an additional carbon source and 1% ammonium sulfate as a nitrogen source. Furthermore, the highest production of avicelase occurs with incubating at 30⁰C with adjusting initial pH at 6.0 and 2 ml of inoculum (106 spores/ml). By studying some factors affecting the enzyme activity, it revealed that the produced avicelase gave the highest activity at 50⁰C and pH 6.0, furthermore the enzyme has good pH stability in acidic conditions and high thermal stability at 50⁰C.

DOI

10.21608/jacb.2020.128901

Keywords

Aspergillus flavus, Avicelase, solid, state fermentation

Authors

First Name

M.

Last Name

Selim

MiddleName

A. E.

Affiliation

Microbiology Department, Faculty of Agriculture, Mansoura University, Egypt

Email

maalawady@mans.edu.eg

City

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Orcid

-

First Name

Sabrien

Last Name

Awad

MiddleName

M. A.

Affiliation

Botany Department, Faculty of Science, Mansoura University, Egypt.

Email

-

City

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Orcid

-

First Name

G. M.

Last Name

Abdel-Fattah

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Mansoura University, Egypt.

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-

City

-

Orcid

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Volume

11

Article Issue

11

Related Issue

18947

Issue Date

2020-11-01

Receive Date

2020-11-01

Publish Date

2020-11-01

Page Start

319

Page End

326

Print ISSN

2090-3626

Online ISSN

2090-3707

Link

https://jacb.journals.ekb.eg/article_128901.html

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https://jacb.journals.ekb.eg/service?article_code=128901

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1

Type

Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

Using Clover Husk for Avicelase Production from Aspergillus flavus Via Solid-State Fermetnation

Details

Type

Article

Created At

22 Jan 2023