This research was carried out in Microbiology Department, Faculty of Agriculture, Damietta University, Damietta, Egypt.Water samples were collected from different sites at Damietta City for both microbiological and physico-chemical examinations during winter 2018. The microbiological results showed that virally investigated types including Poliovirus type 1, Rotavirus, HAV type IB, and H9N2 avian influenza were absent in all examined samples during winter 2018. The highest mean values of total bacterial and total fungal counts in SD and SA sites were 251.333 and 112.667cfu/ml, respectively. There was no bacterial growth on MacConkey broth, Salmonella Shigella (SS) and Staph-110agar media. Algae in the SA and SD sites showed the highest quantities, both motile and non-motile species were found. There was no species of protozoa in all examined sites except SA and SD sites which showed few motile ones. Obtained results of physico-chemical parameters, showed the pH values varied between 7.46 in AF site and 7.96 in SD site. Temperature also, varied between 21 and 22.4°C in EA and SD sites. The highest values of COD and BOD₅²⁰ were in SeA and SD sites and were 9.6 and 18.9 mgO₂/L, respectively. In case of heavy metals, results showed that the highest values of aluminum, cadmium, iron, nickel, lead, manganese, zinc, copper and chromium were in FD,FA,SD,SD,EA,SA, SD,SeA and SD sites and were 0.13,0.019,0.117,0.054,0.046,0.030,0.387,0.007 and0.014 ppm, respectively. So, these results proved that water sources are in agreement with the ES for using of this water for human purposes.