Significant reduction in tomato yield and its fruit quality occurs annually in Egypt during winter and early summer seasons as a result of chilling stress. In this study, a set of tomato lines and cultivars were estimated against their response to treatment by ATP and glycine betaine, in order to select the most promising genotypes for further breeding programs. The Field experiment was carried out at Genetics Department farm, Faculty of Agriculture, Mansoura University during the winter season of 2017/2018. A Randomized Complete Blocks Design in three replicates was adopted in current investigation. The Data were recorded on different vegetative, fruit qualityand yield component traits. The results showed that the Edkawy cultivar had the most useful yield components parameters affected by the exogenous treatment followed by S. Marmande and the line KL-45. This cultivar was characterized by a large number of fruits per inflorescence and average fruit weight around 90 grams. The genetic diversity using SCoT marker technique between 11 tomato genotypes was conducted for selecting the promising genotypes for further breeding programs. The SCoT primers produced a total of 94 bands with an average 11.75 bands per primer ranged from seven (SCoT-6 and SCoT-9) to 22 (SCoT-1) per primer. Among them 84 bands were polymorphic. The highest molecular distance was among KL-41 and KL-03 (0.482). While the lowest MD was between Floradade and Edkawy (0.215). The 11 genotypes showed 14 marker loci, these markers were spread over these genotypes variously differentiating each genotype from the other.