The present study was carried out to evaluate ten genotypes of mung bean for ten important agricultural characteristics. Ten mung bean genotypes were obtained from Bahteem Research Station of the Field Crop Research Institute in Gizain 2019. The complete randomized block design was performed with four replicates, the best genotype selection was made on the basis of ten important agricultural characteristic. Data were recorded for, plant height (cm), branches' number per plant, number of fertile nods per plant, number of pods per nods, pods length (cm), number of seeds per pod, number of pods per plant, number of seeds per plant, seed weight per plant (g) and 100-seed weight (g). The results indicated that there were slightly important differences between the genotypes and genetic diversity was observed for the studied traits and thus, there is an opportunity for genetic improvement. Molecular markers analysis evaluated using Inter Simple Sequence Repeat (ISSR) with ten primers were used on ten mung bean genotypes. A high level of polymorphism was found, where 65 polymorphic bands and 52 monomorphic bands were detected out of total 117 bands. The results showed that there is a high degree of genetic similarity ranged between 0.88 and 0.76 where the highest was 0.88 between genotypes 1, 2 and the least was 0.76 between genotypes 4, 5 and 2, 9.