During the last three decades of sugar beet breeding program has been started in Egypt by several Egyptian investigators and breeders, in the Agricultural Research Station, Alexandria, Sugar Crops Research Institute, Agricultural Research Center, Egypt. The data were very encouragement; breeding materials were introduced for this program from different countries. Twenty sugar beet breeding materials were chosen and cytologically examined to determine chromosome number to employ these breeding materials in hybridization and improvement the prospective genotypes. This study also includes some yield component characters (root weight and sucrose percentage) and 100 seeds weight for all studied breeding materials to make all knowledge of them available for sugar beet breeding program. The results indicated that there are thirteen sugar beet genotypes have diploid chromosome numbers 2n = 2x = 18 (C31/6, C12T, C39, C92, C4612, C790-68H26, Sp-220, Sp-270, Sp-3915, Mo140, Gif. 7, Gif. 8 and Gif. 9), while seven of them have tetraploid chromosome number 2n = 4x = 36 (El-Kasr, D2-R18, D2-R66, D2-R811, C11-R48, C11-R540 and C11T). In root weight character the highest value was found in diploid genotype (C92), 1.530 Kg, while the lowest value in tetraploid genotype (C11T), 0.700 Kg. In sucrose percentage character highest value was found in diploid genotype (Gif. 9), 19.6 %, while the lowest values was found in diploid genotype (C4612), 17.4 %. In 100 seeds weight character highest value was found in tetraploid genotype (D2-R811) with mean value of 2.55gm., while, lowest one was found in diploid genotype (C92), with a mean value of 1.43 gm.