he effect of seedling inoculation after 2 months of cultivation with asymbiotic N₂-fixers only, or asymbiotic N₂-fixers and either organic fertilizer, or with half normal dose of inorganic N-fertilizer on the growth of Basil plant (Ocimum basilicum) and active constituents (volatile oils) were investigated. The experiment was done during the season in the field of experiments in sandy farm soil of Applied Research Center of Medicinal Plants (ARCMP) related to the National Organization for Drug Control and Research (NODCAR).                                                                   .                          The asymbiotic N₂-fixing bacteria used were Azotobacter chroococcum and Azospirillum lipoferum which were isolated from the rhizosphere of Basil plants. The highest densities of Azotobacter chroococcum and Azospirillum lipoferum were found in the rhizosphere of Basil plants, which were inoculated with the active local strains of Azotobacter and Azospirillum respectively in the presence organic fertilizer after four months of cultivation.                        .                                                                                          Data also showed that the growth of Basil plants and their active constituents were positively influenced by seedling inoculation with the asymbiotic N₂-fixers with organic  fertilizer, the highest growth of fresh weight of plant were 2039.5g plant ^-1 and the highest amount of volatile oil being 0.73 ml/100g fresh weight of plant. On the other hand, the un-inoculated controls gave 1417.0g fresh weight plant^-1 and 0.38ml volatile oil/100gm fresh weight of plant after 6 months of cultivation