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89424

DETECTION OF Brucella melitensis FROM FILTERED BOVINE PERIPHERAL-BLOOD BY POLYMERASE CHAIN REACTION TEST

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Last updated: 24 Dec 2024

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Abstract

Brucella melitensis contaminants of bovine peripheral-blood can be concentrated and isolated by filtration; and their DNA can be released for subsequent PCR detection by heating the isolated colony at 95°C for 10 min. Detecting rate of brucellosis in the infected sample was less than 20 CFU/ml of blood, after leukocytes filtration and culturing, as well as on isolated colonies. This PCR method also enables us to make the diagnosing of brucellosis for the animals with a negative complement fixation test.

DOI

10.21608/jacb.2010.89424

Keywords

Blood, Brucella melitensis, Leukocytes, PCR

Authors

First Name

Buthaina

Last Name

Al-Ashkar

MiddleName

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Affiliation

Dept. of Plant Biology, Faculty of Science, University of Damascus

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Orcid

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First Name

A.

Last Name

Al-Mariri

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Affiliation

Dept. of Molecular Biology and Biotechnology, Atomic Energy Commission, Damascus

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Orcid

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First Name

Ibtissam

Last Name

Hamad

MiddleName

-

Affiliation

Dept. of Environment, Faculty of Science, University of Damascus

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Volume

1

Article Issue

4

Related Issue

13444

Issue Date

2010-04-01

Receive Date

2010-04-10

Publish Date

2010-04-01

Page Start

229

Page End

234

Print ISSN

2090-3626

Online ISSN

2090-3707

Article File

JACB_Volume 1_Issue 4_Pages 229-234.pdf

PDF . 356.1kB

Link

https://jacb.journals.ekb.eg/article_89424.html

Detail API

https://jacb.journals.ekb.eg/service?article_code=89424

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3

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Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

DETECTION OF Brucella melitensis FROM FILTERED BOVINE PERIPHERAL-BLOOD BY POLYMERASE CHAIN REACTION TEST

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Article

Created At

22 Jan 2023