Three spindle fiber inhibitors (8-hydroxyquinoline, para-dichlorobenzene and colchicine) at four concentrations were used in sugar beet (Beta vulgaris L.) plants to induce polyploidy. The aberrations were recorded and recognized in root tip and young leave cells. The results obtained showed that different types of aberrations were observed. These types are fragmentation, ring shape, stickiness, gaps, bridge, cell in lyses and end to end association. Different ratios were recorded for the three tested compounds. However, colchicine was proven to induce the minimum ratio of aberrations and to be the effective compound in causing polyploidy compared with other compounds.