Sugarcane juice sub- products was the main source of carbohydrates for ethanol productivity and biomass formation . It contains sugars as a good alternative material for ethanol production from yeast to meet the large scale consumption of petroleum fuels . Therefore, the main objective of this study was undertaken to determine the correlation between ethanol productivity and biomass formation using different concentrations of sugarcane juice industrial by - products as a main source of carbon .15 hybrids of Saccharomyces cerevisiae derived from three crosses conducted between five parental strains were used in this study . The results of fermentation process on bioethanol production were graphically diagrammatic. The data showed in most cases negative correlations between ethanol production and biomass formation as shown from genotypes of crosses P₁ x P₄ and P₃ x P₅ . This indicated that high productivity of ethanol was associated with a lower biomass formation due to ethanol toxicity on the viability of yeast cells . On the contrary , genotypes resulted from cross between P₂ x P₅ showed positive correlation between both parameters at 0.02 and 0.06 g of sugarcane juice sub - products . Therefore, it is possible that genotypes derived from this cross may be more tolerant to ethanol inhibitor. In addition , the greater use of sugars for growing yeast cells resulting in a lower ethanol productivity and vice – versa . The results indicated that genome shuffling via hybridization is a powerful tool for inducing genetic recombinants in yeast for desirable industrial phenotypes.