The point of this investigation was to decide the hereditary inconstancy and break down the connection between four faba bean cultivars and six promising lines utilizing inter simple sequence repeats (ISSR) markers. Faba bean genotypes were gathered by their resistance to the bean yellow mosaic virus infection. With the acquired band registration, a paired data matrix was built to perform the comparing statistical analysis. The used ISSR markers created 148 fixed and repeatable bands, of which 75 were polymorphic. The values of resolution power (Rp), polymorphic information content (PIC), and marker index (MI), uncovered that primer ISSR-14 was the most effective to break down hereditary changeability with estimations of 5.8, 0.80, and 6.40, respectively, followed by primers ISSR-6 and ISSR-18. Genetic distances swayed somewhere in the range of 0.78 and 0.91, and checked the groupings saw in the dendrogram, which demonstrates high change at the degree of DNA among the investigated genotypes, watching six characterized gatherings as indicated by UPGMA examination. In the examination of fundamental segments, the enrolled groupings were dictated by the source of the origin of the gathering. The utilization of ISSR markers was effective to describe at the degree of DNA the evaluated bean genotypes, showing the presence of inconstancy, the recognized differentiating promotions can be used in hereditary improvement projects planned for settling the requirements of the producers.