This study was conducted at RiceResearch & TrainingCenter, Sakha, Kafr El-Sheikh, Egypt during 2007, 2008 and 2009 growing seasons. Seventeen genotypes were used including three CMS lines, three restorers, nine hybrids and two Egyptian checks (Egyptian yasmine as a highly susceptible and Sakha 101 as a resistant genotype) and grown in a randomized complete block design with three replications. The main objective of this study is to identify the genetical and biochemical bases of rice resistance against rice stem borer. The results revealed that the high values of infestation were detected in rice genotypes having low phosphorus, dry matter and silica contents. In contrast, the genotypes that showed low infestation had the highest contents of phosphorus, dry matter and silica. On the other hand, potassium, pH values and plant moisture contents were positively correlated with the infested stems. The estimate of variance due GCA was higher than that due to SCA for all traits suggesting greater importance of additive genetic variance. However, breeders and entomologists should select rice entries with high silica, phosphorus and dry matter contents to reduce feeding capacity of  stem borer larva.