The rice stem borer (Chilo agamemnon Bles.) is one of the most devastating insects of rice which reducing rice yield in Egypt. The use of resistant rice genotypes remains one of the most reliable methods to control this insect. This investigation aimed to study the mechanisms of resistance (antixenosis, antibiosis and tolerance) in seventeen rice genotypes were evaluated under greenhouse and field conditions. Level of larvae feeding on rice plant was one of the parameter for antixenosis, while weight of larvae or pupae were a measure of antibiosis. Percentage of dead hearts and white heads were used for estimating the tolerance to rice stem borer. The results showed that the hybrid: IR 58025A x Giza 178 R had the highest larval survival percentage, larval weight, pupal weight, dead hearts and white heads. Generally, the hybrid IR 58025A x Giza 178 R and Sakha 101 were the most susceptible and tolerant genotypes, respectively. The tolerant genotypes against stem borer, should have high number of tillers, narrow stem diameter and flag leaf angle. These genotypes could be considered as considers in breeding program to develop new plant type unsuitable for larva stem borer living.