Root rot disease, caused by Fusarium solani f. sp. phaseoli, is one of the main root diseases impacting production of common bean in Egypt. The antagonistic effects of Bacillus pumilus and Trichoderma viride, were tested against F. solani, in vitro and in greenhouse conditions. In vitro tests, B. pumilus and T. viridesignificantly reduced the mycelial growth of pathogenic fungi. In greenhouse experiment, B. pumilus and T. viride, as soil treatments, significantly reduced the pre and post-emergence damping off disease incidence of F. solani under artificial infection. The percentages of disease incidence in treated plants ranged from 26.2 to 30.0%, compared to 72.0% in control plants, in both pre and post-emergence stages, respectively. The best protection of damping off disease was obtained by T. viride, followed by B. pumilus . All treatments improved the survival plant and growth parameters. Results showed increased levels of peroxidase and polyphenoloxidase activities in treated bean plants, compared to untreated ones.