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55014

SYMBIOTIC NITROGEN FIXATION OF FABA BEAN PLANTS INOCULATED WITH SALT-TOLERANT Rhizobium ISOLATES

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Last updated: 04 Jan 2025

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Abstract

          Rhizobium leguminosarum biovar viciae isolated from salt-affected faba bean fields were in vitro tested.  Different isolates were found to be varied in their tolerance to salinity. F1 and E1 were found to be the most salt-tolerant isolates even at EC-level of 12 ds m-1. Seeds of Nubaria 1 cv. were inoculated with either F1 or E1, in comparison with a less salt-tolerant one (F9) in pots their salinity were adjusted at 0, 3, 6, 9 and 12 ds m-1 under aseptic conditions. Number and dry weight of nodules were decreased strongly by increasing salinity levels in comparison with control. Significant enhancement of N % accumulated in the shoots was also noticed due to the biological inoculation with both Rhizobium isolates in presence or absence of salinity. Under non-aseptic conditions, efficacy of F1 and E1 with the effect of mineral N-supply (25 and 100 %) was compared. In addition to Nubaria 1 cv., Sakha 1 cv. was also used in the evaluation process for N-fixation at 0, 6, 9 ds m-1 levels. Number of nodules formed on Nubaria 1 at 9 ds m-1 was highly increased from 7.86 and 5.44 for 25 and 100 % N-supply to 82.33 and 93.33 for F1 and E1, respectively. Accordingly, nodular dry weights were also increased to 0.40 and 0.41 g plant-1 for F1 and E1 instead of 0.13 and 0.08 g plant-1 for 25 and 100 % N, respectively. The beneficial effects of F1 and E1 inoculation were also extended to enhance N % of shoots and seed yield parameters. Therefore, the symbiotic N2-fixation process has a great potential to improve growth and productivity of faba bean plants in comparison with the mineral N-supply to overcome the harmful effects of salinity.

DOI

10.21608/jacb.2012.55014

Keywords

Vicia faba, Rhizobium, salinity, mineral N-supply

Authors

First Name

Nagwa M. M.

Last Name

El-Khateeb

MiddleName

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Affiliation

Dept. of Agric. Botany, Agric. Microbiology, Faculty of Agric., Kafrelsheikh Univ., Egypt

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Orcid

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First Name

M. E.

Last Name

Shalaby

MiddleName

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Affiliation

Dept. of Agric. Botany, Agric. Microbiology, Faculty of Agric., Kafrelsheikh Univ., Egypt

Email

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City

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Orcid

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First Name

E. B. A.

Last Name

Belal

MiddleName

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Affiliation

Dept. of Agric. Botany, Agric. Microbiology, Faculty of Agric., Kafrelsheikh Univ., Egypt

Email

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City

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Orcid

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First Name

SH. M. A.

Last Name

El-Gremi

MiddleName

-

Affiliation

Dept. of Agric. Botany, Agric. Microbiology, Faculty of Agric., Kafrelsheikh Univ., Egypt

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Volume

3

Article Issue

10

Related Issue

8399

Issue Date

2012-10-01

Receive Date

2012-10-13

Publish Date

2012-10-01

Page Start

411

Page End

423

Print ISSN

2090-3626

Online ISSN

2090-3707

Link

https://jacb.journals.ekb.eg/article_55014.html

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https://jacb.journals.ekb.eg/service?article_code=55014

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2

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Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

SYMBIOTIC NITROGEN FIXATION OF FABA BEAN PLANTS INOCULATED WITH SALT-TOLERANT Rhizobium ISOLATES

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Article

Created At

22 Jan 2023