Embryonic stem cells have the amazing ability to differentiation into all types cell of the three germ layers, if a suitable environment for its, and it have great potential for tissue engineering application. Embryonic stem cells, replacement therapy-cell therapy- is one of the most promising treatment for neurodegerative disease such as Parkinson`s disease. Cultured two cell line derived from the inner cell mass of murine blastocyst. Cell line: 1- OCT4- GFP.  2- SOX1- GFP. Cultured murine embryonic stem cells in DMEM medium or GMEM medium with leukaemia inhibitory factor (LIF),has also been cultured in these medium without LIF and added the differentiation factors for differentiation into neural cells. Cultured cells were peaceful , healthy and good, therefore some cells were then placed in liquid nitrogen, but at one stage cell were contaminated, contaminated cells were sterilized and discarded. Conservation and cultivation technology requires effort, hard work and full knowledge to keep the cells on their health , safety ,quality and thus the possibility for conservation that cells in culture and keep it in liquid nitrogen. The some applies to the possibility of differentiation into neural cells, after withdrawal inhibitory factor, has beensaid all the application for that, to in order to benefit every researcher, especially locally, in the area.