Leaf rust caused by the biotrophic fungus Puccinia triticina, is one of the most important diseases of worldwide countries and Egypt. The use of resistance genes is of a great interest in numerous breeding programs. Our objectives were to determine the number of resistance gene pairs to wheat leaf rust present in eleven crosses derived from two low rust severity wheat cultivars i.e. Gemmeiza 9 and Sakha 94 with six and five high rust severity wheat cultivars, respectively, at both seedling in greenhouse and at adult plant stages under field conditions. The obtained results showed a high effective response against artificial and natural infection of wheat leaf rust at adult plant than these recorded at seedling stage. The study of combination between traditional wheat cultivars of Egyptian origin showing durable resistance proved to have a great value since they can provide new sources of resistance to such a disease.