This study was undertaken to determine some selected nutritive chemical composition of dried mulberry fruits and leaves (proximate composition: moisture, ash, fiber, protein, fat and carbohydrate, amino acids and minerals) and evaluation of antimicrobial and anticancer potential of their methanolic extract. The results indicated that the leaves have more nutritious quality than the fruits. The dried mulberry leaves recorded higher content of ash, protein, fiber, amino acids and minerals than that in fruits while moisture and fat content were higher in fruits than in leaves. The carbohydrate content was slightly higher in fruits than in leaves. However, both of them were nutritionally rich. The antimicrobial effect of different concentrations (5, 10 and 15 mg/ml DMSO) of mulberry fruits and leaves extract on growth and survival of Staphylococcus aureus strain and Escherichia coli strain in vitro and in mulberry juice were evaluated. Both fruits and leaves extract have attenuated effect on both bacteria. The concentration, 15 mg/ml of mulberry fruits extract represent the optimum concentration for decreasing E. coli and Staph. aureus counts in liquid medium such paid to decreasing them from 5X10¹⁰  and 5X10¹² to 14X10² and 4X10⁵ cfu/ml respectively. The different concentrations (10 and 15 mg/ml) of fruits and leaves extract induced completely elimination of Staph. aureus from mulberry juice and reduction of E. coli from 5X10¹⁰ to 4X10² and 3X10³ cfu/ml  respectively at concentration 15 mg/ml. The expression of p53 (tumor suppressor gene) from three types of cancer cell lines (Hep-2 (Larynx carcinoma), HepG2 (liver carcinoma) and CaCo2 (colorectal adenocarcinoma)) treated with fruits and leaves extract were evaluated to explore their anticancer effect. The results showed that the cancer cells treated with fruits and leaves extract were negative for p53 gene expression as the gene not detected comparing with positive cell control. The tested extracts were not anticancer agent.