Response of three varieties of sweet sorghum (Sorghum bicolor) namely: Hunny, Brandies and Prawel, to callus induction, embryogenic production, and in vitro salt tolerance were studied. In addition electrophoretic patterns of proteins and peroxidase isozyme patterns were studied to evaluate the response the obtaining calli of sweet sorghum to salt treatments with different concentrations of NaCl. The results obtained showed differential effect among the tested varieties. Brandies variety showed high response for callus induction, callus weight and salt tolerance comparing with other varieties.The present results might be useful in evaluative purposes in breeding program.