Ten F₄ families with the highest  grain yield/plant were selected from cross between two cultivars (long spike-58 × Giza -168) and evaluated for tolerance to salinity stress conditions. Crosses were also made between ten selected plants. In the second cycle of selection, F₅ families were classified into two groups according to their performance of some salinity stress related traits  such as grain yield and stress physiological traits. Significant positive response to selection for grain yield was ranged from 69.94% of means to 135.83% for intra cross between selections within population. The indirect response to selection ranged from 108.34% for proline content to 42.34% in chlorophyll content. The results indicated the presence of three positive and four negative RAPD markers that could be considered as reliable markers for salinity tolerance in bread wheat.