A bacterium isolated from soil sample contaminated by old tire rubber in Qena governorate, Egypt. It was shown to be able to utilize natural rubber (NR) latex, synthetic poly (cis-1,4-isoprene) and other isopronoid compounds as a sole carbon source and energy. The strain was aerobic, Gram-positive and produced very short elementary branching hyphe which disintegrated into rod/cocci-like elements. Taxonimic characterization of this isolate by 16S rDNA gene sequence analysis  showed highest similarities to the 16S rDNA gene sequence of Gordonia alkanivorans (99,8%), which is mesophilic actinomycetes. Bacterial isolate was, therefore, referred to as Gordonia alkanivorans strain E1. Gordonia alkanivorans strain E1 grows well at 25, 30, and 35 °C but it did not grow at 45 °C. It was able to utilize a wide variety of carbon source such as glucose, fructose, hexadecane, acetate, starch and skim milk.  Degradation behaviour indicated that the strain grow adhesively and depends on direct contact with the rubber substrate so belongs to CMN-group (Corynebacterium, Mycobacterium, Nocardia). The capability of rubber degradation was confirmed by mineralization experiments. Degradation of isoprenoid compounds and related compounds namely, squalane, squalene, phytol, acetonylacetone, geranylacetone citronellal and citronellic acid was recognized indicating that the bacterium has the metabolic capability to utilize some isoprenoid compounds as carbon and energy sources.