The present study was carried out to find out the antimicrobial activity of ethyl acetate , acetone ,ethanol and petroleum ether of lemon (Citrus lemone L) ,mandarin(Citrus reticulata L.) and orange(Citrus sinensis L.) peel at different concentrations (5,10,25,50 mg/ml) antimicrobial analysis were done using disc diffusion technique and tube dilution method against E.coli O₁₅₇: H ₇ Staphylococcus aureus Pseudomonas flourescens , Pseudomonas aeruginosa, Candida albicans and the results compared with the standard antibiotics ,e.g penicillin ,chloamphincol , erythromycin and chlorotetracycline The results showed that, the extracts at different concentrations  (25,50 mg/ml) of lemon ,mandarin and orange peel exhibited the maximum zones of inhibition against candida albicans by using disc diffusion technique. On the other hand density least of the bacterial growth was obtained by using tube dilution method at 10 mg/ml concentration of different extracts against each E.coli O₁₅₇:H₇ and Staphylococcus aureus.As showed candida albicans not sensitive for all tested antibiotics.