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SEMI QUANTITATIVE RT PCR ANALYSIS OF TWO SILICON TRANSPORTER GENES IN SILICON UPTAKE AND STEM BORER RESISTANCE IN SOME RICE GENOTYPES

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Last updated: 04 Jan 2025

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Abstract

       Relative gene expression of two Si transporter genes (Lsi1 and Lsi2) was examined in the roots of seven tested rice genotypes grown in solution culture (9 days old) using semi‑quantitative RT‑PCR technique. The Si concentration in roots, stems and shoots of these rice accessions (30 days old grown in solution culture) was measured. Moreover, stem diameter, white head percentage and plant Si content were studied at the heading stage for the tested rice genotypes grown in field in order to insure stem borer resistance trait in the higher Si content and lower stem diameter rice plants. The results of this study indicate the direct association of the level of gene expression of Si transporter genes and plant Si content and its associated characters with stem borer resistance trait in rice.

DOI

10.21608/jacb.2015.48413

Authors

First Name

M. E.

Last Name

Eldenary

MiddleName

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Affiliation

Department of Genetics, Faculty of Agriculture,Tanta University, Egypt.

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Volume

6

Article Issue

8

Related Issue

7530

Issue Date

2015-08-01

Receive Date

2015-08-08

Publish Date

2015-08-01

Page Start

285

Page End

292

Print ISSN

2090-3626

Online ISSN

2090-3707

Link

https://jacb.journals.ekb.eg/article_48413.html

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https://jacb.journals.ekb.eg/service?article_code=48413

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2

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Original Article

Type Code

883

Publication Type

Journal

Publication Title

Journal of Agricultural Chemistry and Biotechnology

Publication Link

https://jacb.journals.ekb.eg/

MainTitle

SEMI QUANTITATIVE RT PCR ANALYSIS OF TWO SILICON TRANSPORTER GENES IN SILICON UPTAKE AND STEM BORER RESISTANCE IN SOME RICE GENOTYPES

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Article

Created At

22 Jan 2023